pbabe krasg12d plasmid Search Results


pbabe puro krasg12d  (Addgene inc)
93
Addgene inc pbabe puro krasg12d
Pbabe Puro Krasg12d, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/pBabe-Kras+G12D+(Plasmid+%2358902)/pm41241302-47-6-11
Average 93 stars, based on 1 article reviews
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krasg12d  (Addgene inc)
93
Addgene inc krasg12d
Krasg12d, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/H516+SONIC+KrasG12D-IRES-luciferase+donor+(Plasmid+%23138178)/pm36385525-370-0-5
Average 93 stars, based on 1 article reviews
krasg12d - by Bioz Stars, 2026-09
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pbabe puro  (Addgene inc)
96
Addgene inc pbabe puro
Pbabe Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/pBABE-puro+(Plasmid+%231764)/pm36385525-370-4-5
Average 96 stars, based on 1 article reviews
pbabe puro - by Bioz Stars, 2026-09
96/100 stars
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pbabe kraswt plasmid  (Addgene inc)
93
Addgene inc pbabe kraswt plasmid
Pbabe Kraswt Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/pBabe-Kras+Wt+(Plasmid+%2375282)/10__1158_slash_1078___0432__ccr___17___3438-79-12-15
Average 93 stars, based on 1 article reviews
pbabe kraswt plasmid - by Bioz Stars, 2026-09
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kras g12c  (Addgene inc)
93
Addgene inc kras g12c
<t>KRAS</t> positively regulates MTH1 expression (A) The indicated retroviral control empty vector (EV) and KRAS constructs were stably transduced into H1563 cells. Immunoblotting for MTH1, KRAS and loading is shown. Higher mobility bands reflect constructs with an N-terminal HA-tag. Two different exposures are shown as the variance in KRAS expression across the different constructs alters the linear ranges for their respective signals, saturating the differences among the mutant KRAS signals at exposures required to highlight differences between the control and wt KRAS expression construct. (B) Oncogenic KRAS significantly elevates MTH1 expression. Quantitation of MTH1 expression from the indicated transduced variants in (A) shown on the x-axis. Error bars represent the SEM with p-values calculated via an unpaired Student's t-test. (C) Depletion of KRAS oncoprotein decreases MTH1 levels. Immunoblotting for MTH1, KRAS levels and loading control GAPDH in H358 cells stably transduced with shKRAS or control shGFP lentiviral constructs. (D) Inhibition of KRAS activity decreases MTH1 levels. Immunoblotting for MTH1 levels in H358 cells treated for 24 h with 0.1 μM of the KRAS <t>G12C-specific</t> inhibitor, AMG-510. (E) MTH1 expression is not regulated by KRAS through the ETS1 transcriptional element. KRAS G12C LUAD cell lines, H23 and H358, were stably transduced with lentiviral shGFP or shETS1 constructs. Immunoblotting of harvested cells shows MTH1 levels are not affected by ETS1 knockdown. (F) MTH1 expression in wt KRAS H1563 cells is increased by oxidants. Cells were treated with the indicated concentrations of H 2 O 2 , erastin or menadione for the indicated timepoints. Immunoblotting of H1563 total protein lysates for MTH1, phosphorylated (p) and total (t) AKT and ERK1/2 is shown. Tubulin is shown as the loading control. For duplicate blots run on the same set of lysates, tubulin signal is shown for each set of bands run on the same gel.
Kras G12c, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/pBabe-Kras+G12C+(Plasmid+%2358901)/pmc11999683-60-4-7
Average 93 stars, based on 1 article reviews
kras g12c - by Bioz Stars, 2026-09
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plko 1 vector  (Addgene inc)
96
Addgene inc plko 1 vector
<t>KRAS</t> positively regulates MTH1 expression (A) The indicated retroviral control empty vector (EV) and KRAS constructs were stably transduced into H1563 cells. Immunoblotting for MTH1, KRAS and loading is shown. Higher mobility bands reflect constructs with an N-terminal HA-tag. Two different exposures are shown as the variance in KRAS expression across the different constructs alters the linear ranges for their respective signals, saturating the differences among the mutant KRAS signals at exposures required to highlight differences between the control and wt KRAS expression construct. (B) Oncogenic KRAS significantly elevates MTH1 expression. Quantitation of MTH1 expression from the indicated transduced variants in (A) shown on the x-axis. Error bars represent the SEM with p-values calculated via an unpaired Student's t-test. (C) Depletion of KRAS oncoprotein decreases MTH1 levels. Immunoblotting for MTH1, KRAS levels and loading control GAPDH in H358 cells stably transduced with shKRAS or control shGFP lentiviral constructs. (D) Inhibition of KRAS activity decreases MTH1 levels. Immunoblotting for MTH1 levels in H358 cells treated for 24 h with 0.1 μM of the KRAS <t>G12C-specific</t> inhibitor, AMG-510. (E) MTH1 expression is not regulated by KRAS through the ETS1 transcriptional element. KRAS G12C LUAD cell lines, H23 and H358, were stably transduced with lentiviral shGFP or shETS1 constructs. Immunoblotting of harvested cells shows MTH1 levels are not affected by ETS1 knockdown. (F) MTH1 expression in wt KRAS H1563 cells is increased by oxidants. Cells were treated with the indicated concentrations of H 2 O 2 , erastin or menadione for the indicated timepoints. Immunoblotting of H1563 total protein lysates for MTH1, phosphorylated (p) and total (t) AKT and ERK1/2 is shown. Tubulin is shown as the loading control. For duplicate blots run on the same set of lysates, tubulin signal is shown for each set of bands run on the same gel.
Plko 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/pLKO%2E1+(Plasmid+%238407)/10__1128_slash_mcb__00079___16-19-27-29
Average 96 stars, based on 1 article reviews
plko 1 vector - by Bioz Stars, 2026-09
96/100 stars
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krasq61h retroviral plasmids  (Addgene inc)
93
Addgene inc krasq61h retroviral plasmids
<t>KRAS</t> positively regulates MTH1 expression (A) The indicated retroviral control empty vector (EV) and KRAS constructs were stably transduced into H1563 cells. Immunoblotting for MTH1, KRAS and loading is shown. Higher mobility bands reflect constructs with an N-terminal HA-tag. Two different exposures are shown as the variance in KRAS expression across the different constructs alters the linear ranges for their respective signals, saturating the differences among the mutant KRAS signals at exposures required to highlight differences between the control and wt KRAS expression construct. (B) Oncogenic KRAS significantly elevates MTH1 expression. Quantitation of MTH1 expression from the indicated transduced variants in (A) shown on the x-axis. Error bars represent the SEM with p-values calculated via an unpaired Student's t-test. (C) Depletion of KRAS oncoprotein decreases MTH1 levels. Immunoblotting for MTH1, KRAS levels and loading control GAPDH in H358 cells stably transduced with shKRAS or control shGFP lentiviral constructs. (D) Inhibition of KRAS activity decreases MTH1 levels. Immunoblotting for MTH1 levels in H358 cells treated for 24 h with 0.1 μM of the KRAS <t>G12C-specific</t> inhibitor, AMG-510. (E) MTH1 expression is not regulated by KRAS through the ETS1 transcriptional element. KRAS G12C LUAD cell lines, H23 and H358, were stably transduced with lentiviral shGFP or shETS1 constructs. Immunoblotting of harvested cells shows MTH1 levels are not affected by ETS1 knockdown. (F) MTH1 expression in wt KRAS H1563 cells is increased by oxidants. Cells were treated with the indicated concentrations of H 2 O 2 , erastin or menadione for the indicated timepoints. Immunoblotting of H1563 total protein lysates for MTH1, phosphorylated (p) and total (t) AKT and ERK1/2 is shown. Tubulin is shown as the loading control. For duplicate blots run on the same set of lysates, tubulin signal is shown for each set of bands run on the same gel.
Krasq61h Retroviral Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/Kras+(G12V)-pcw107+(Plasmid+%2364546)/pmc10199551-55-15-33
Average 93 stars, based on 1 article reviews
krasq61h retroviral plasmids - by Bioz Stars, 2026-09
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shrna sequence  (Addgene inc)
96
Addgene inc shrna sequence
<t>KRAS</t> positively regulates MTH1 expression (A) The indicated retroviral control empty vector (EV) and KRAS constructs were stably transduced into H1563 cells. Immunoblotting for MTH1, KRAS and loading is shown. Higher mobility bands reflect constructs with an N-terminal HA-tag. Two different exposures are shown as the variance in KRAS expression across the different constructs alters the linear ranges for their respective signals, saturating the differences among the mutant KRAS signals at exposures required to highlight differences between the control and wt KRAS expression construct. (B) Oncogenic KRAS significantly elevates MTH1 expression. Quantitation of MTH1 expression from the indicated transduced variants in (A) shown on the x-axis. Error bars represent the SEM with p-values calculated via an unpaired Student's t-test. (C) Depletion of KRAS oncoprotein decreases MTH1 levels. Immunoblotting for MTH1, KRAS levels and loading control GAPDH in H358 cells stably transduced with shKRAS or control shGFP lentiviral constructs. (D) Inhibition of KRAS activity decreases MTH1 levels. Immunoblotting for MTH1 levels in H358 cells treated for 24 h with 0.1 μM of the KRAS <t>G12C-specific</t> inhibitor, AMG-510. (E) MTH1 expression is not regulated by KRAS through the ETS1 transcriptional element. KRAS G12C LUAD cell lines, H23 and H358, were stably transduced with lentiviral shGFP or shETS1 constructs. Immunoblotting of harvested cells shows MTH1 levels are not affected by ETS1 knockdown. (F) MTH1 expression in wt KRAS H1563 cells is increased by oxidants. Cells were treated with the indicated concentrations of H 2 O 2 , erastin or menadione for the indicated timepoints. Immunoblotting of H1563 total protein lysates for MTH1, phosphorylated (p) and total (t) AKT and ERK1/2 is shown. Tubulin is shown as the loading control. For duplicate blots run on the same set of lysates, tubulin signal is shown for each set of bands run on the same gel.
Shrna Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+krasg12d+plasmid/shRNA+(Plasmid+%2355783)/pm38733583-228-247-268
Average 96 stars, based on 1 article reviews
shrna sequence - by Bioz Stars, 2026-09
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Image Search Results


KRAS positively regulates MTH1 expression (A) The indicated retroviral control empty vector (EV) and KRAS constructs were stably transduced into H1563 cells. Immunoblotting for MTH1, KRAS and loading is shown. Higher mobility bands reflect constructs with an N-terminal HA-tag. Two different exposures are shown as the variance in KRAS expression across the different constructs alters the linear ranges for their respective signals, saturating the differences among the mutant KRAS signals at exposures required to highlight differences between the control and wt KRAS expression construct. (B) Oncogenic KRAS significantly elevates MTH1 expression. Quantitation of MTH1 expression from the indicated transduced variants in (A) shown on the x-axis. Error bars represent the SEM with p-values calculated via an unpaired Student's t-test. (C) Depletion of KRAS oncoprotein decreases MTH1 levels. Immunoblotting for MTH1, KRAS levels and loading control GAPDH in H358 cells stably transduced with shKRAS or control shGFP lentiviral constructs. (D) Inhibition of KRAS activity decreases MTH1 levels. Immunoblotting for MTH1 levels in H358 cells treated for 24 h with 0.1 μM of the KRAS G12C-specific inhibitor, AMG-510. (E) MTH1 expression is not regulated by KRAS through the ETS1 transcriptional element. KRAS G12C LUAD cell lines, H23 and H358, were stably transduced with lentiviral shGFP or shETS1 constructs. Immunoblotting of harvested cells shows MTH1 levels are not affected by ETS1 knockdown. (F) MTH1 expression in wt KRAS H1563 cells is increased by oxidants. Cells were treated with the indicated concentrations of H 2 O 2 , erastin or menadione for the indicated timepoints. Immunoblotting of H1563 total protein lysates for MTH1, phosphorylated (p) and total (t) AKT and ERK1/2 is shown. Tubulin is shown as the loading control. For duplicate blots run on the same set of lysates, tubulin signal is shown for each set of bands run on the same gel.

Journal: Redox Biology

Article Title: Oncogenic KRAS addiction states differentially influence MTH1 expression and 8-oxodGTPase activity in lung adenocarcinoma

doi: 10.1016/j.redox.2025.103610

Figure Lengend Snippet: KRAS positively regulates MTH1 expression (A) The indicated retroviral control empty vector (EV) and KRAS constructs were stably transduced into H1563 cells. Immunoblotting for MTH1, KRAS and loading is shown. Higher mobility bands reflect constructs with an N-terminal HA-tag. Two different exposures are shown as the variance in KRAS expression across the different constructs alters the linear ranges for their respective signals, saturating the differences among the mutant KRAS signals at exposures required to highlight differences between the control and wt KRAS expression construct. (B) Oncogenic KRAS significantly elevates MTH1 expression. Quantitation of MTH1 expression from the indicated transduced variants in (A) shown on the x-axis. Error bars represent the SEM with p-values calculated via an unpaired Student's t-test. (C) Depletion of KRAS oncoprotein decreases MTH1 levels. Immunoblotting for MTH1, KRAS levels and loading control GAPDH in H358 cells stably transduced with shKRAS or control shGFP lentiviral constructs. (D) Inhibition of KRAS activity decreases MTH1 levels. Immunoblotting for MTH1 levels in H358 cells treated for 24 h with 0.1 μM of the KRAS G12C-specific inhibitor, AMG-510. (E) MTH1 expression is not regulated by KRAS through the ETS1 transcriptional element. KRAS G12C LUAD cell lines, H23 and H358, were stably transduced with lentiviral shGFP or shETS1 constructs. Immunoblotting of harvested cells shows MTH1 levels are not affected by ETS1 knockdown. (F) MTH1 expression in wt KRAS H1563 cells is increased by oxidants. Cells were treated with the indicated concentrations of H 2 O 2 , erastin or menadione for the indicated timepoints. Immunoblotting of H1563 total protein lysates for MTH1, phosphorylated (p) and total (t) AKT and ERK1/2 is shown. Tubulin is shown as the loading control. For duplicate blots run on the same set of lysates, tubulin signal is shown for each set of bands run on the same gel.

Article Snippet: The wtKRAS (plasmid#75282; RRID:Addgene_75282), KRAS G12C (plasmid#58901; RRID:Addgene_58901), and KRASG12D (plasmid#58902; RRID:Addgene_58902) plasmids were acquired from Addgene (Cambridge, MA), and were deposited by Dr. Channing Der's laboratory.

Techniques: Expressing, Retroviral, Control, Plasmid Preparation, Construct, Stable Transfection, Western Blot, Mutagenesis, Quantitation Assay, Transduction, Inhibition, Activity Assay, Knockdown